Recently completed projects from the Protein Production Group:

· Site-directed mutagenesis of 3 single-Cys calmodulin mutants
· Subcloning, expression and purification of functional bacterial ferrtin (bfd)
· Subcloning, expression and purification of Rack GTPase
· Cloning, expression and purification of ChxR with cleavable 6-His tag in E coli
· Construction of a GFP-fusion protein using improved overlapping PCR
· Purification of an expressed mammalian membrane protein, the P450 NADPH reductase
· Expression of the human APC protein (310 kD) in E.coli
· Purification of gram-negative bacterial OmpC
· Sample preparation of needle protein from Shigella for Solid State NMR
· Enzymatic assisted refolding of HA fusion protein from inclusion bodies
· Purification of the recombinant yeast Hsp82 in E. coli
· Construction of K244gamma mutant of CF1
· Thrombin cleavage and purification of D4 domain from recombinant GST fusion protein
· Large scale production of 6-His rTEV protease

Example of a gel produced in the Protein Production Group Laboratory.

Dr. Gao is examining results from an experiment conducted on the Biacore 3000 Surface Plasma Resonance system.

COBRE in Protein Structure and Function

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Copyright © 2009
Center of Biomedical Research Excellence in Protein Structure and Function
Department of Medicinal Chemistry, School of Pharmacy
The University of Kansas
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