Biomolecular NMR Laboratory
About the Lab
The COBRE Biomolecular NMR Laboratory maintains two high field NMR spectrometers in support of structural and dynamics studies of biomolecules with COBRE and other investigators in Kansas and the region. Its capabilities include determining high resolution structures, biological macromolecules, elucidation and structural mapping of protein-protein, protein-nucleic acid, protein-peptide, protein-drug interactions, and studies of the dynamics of proteins and their complexes in solution.
Laboratory staff provide advice, consultation, training, assistance and service to investigators. The staff is also responsible for maintenance and upgrades of the two high field NMR instruments, implementation of new NMR pulse sequences, and assisting local and remote users with technical problems.
The laboratory is equipped with a Bruker Avance 800 MHz NMR instrument fitted with a TCI cryoprobe and a Bruker Avance III 600 MHz with a variety of probes. It is located in close proximity to other core service laboratories in the KU Structural Biology Center.
Clients at the Biomolecular NMR Laboratory can now use a recently installed autosampler on the 600 MHz NMR. The autosampler (Bruker BioSpin SampleCaseä) allows a researcher to set up 24 NMR experiments at one time and eliminate periodic intervention by the user. Manual insertion and ejection of samples is possible with the push of a button. The autosampler is located at user height, ensures simple random access automation and eliminates the need for steps or ladders. Direct access to the magnet is not restricted by these sample changers. There is a sensor within the system to eliminate the risk of tube breakage and loss of sample. The system is good for screening small molecules with target protein and 2D-1H-15N-HSQC chemical shift perturbation related studies with many samples.