Protein Structure (X-ray) Laboratory

Major Equipment

  • High brilliance rotating anode generator (Rigaku RU-H3RHB); Blue Max Flux (Osmic) confocal optical system; Two theta stage and inverse Phi axis R-AXIS IV++ imaging plate system;
  • R-AXIS IV++ image plate system; X-stream 2000 cryogenic system
  • Molecular Dimensions crystallization incubator for low temperature crystallization
  • Formulatrix NT8 drop setting robot equipped with plate copy and LCP options
  • Formulatrix Rock Imager 2 (RI2) with UV-VIS imaging, multiwavelength fluorescence imaging
  • Formulatrix RockMaker software with secure web access to user accounts
  • Two Olympus stereo microscopes (CMOS camera for crystal imaging)
  • Eppendorf 5414R and Spectrafuge-16M mini centrifuges for concentrating proteins
  • Rigaku Actor and Universal pucks for shipping to the synchrotron (172 crystals)
  • Millipore ultrapure water filtration system
  • Three refrigerators for sample and buffer storage
  • Five Dell Precision Linux workstations containing modern software for chemical computing analyses, molecular docking, X-ray data processing, structure solution, structure refinement, model building, structure analysis and preparation of publication quality graphics.
NT8 drop setting robot and Rock Imager 2 (RI2) crystal imaging system

The PSL crystallization laboratory offers a:

1) NT8 drop setting robot and 2) Rock Imager 2 (RI2)
crystal imaging system. Since these components are
integrated, it streamlines the crystallization process
and permits easy tracking of all experimental
details and results.

Microscopes available at the Protein Structure Laboratory

Two Olympus stereo microscopes (CMOS camera for
crystal imaging) are available.

High brilliance X-ray generator, Xtream 2000,and the<br />new Raxis IV++ imaging plate

High brilliance X-ray generator, Xtream 2000,and the
new Raxis IV++ imaging plate available at the PSL offer
a complete system forĀ  single crystal diffraction
data acquisition.