Protein Purification Group

Equipment

E. coli and insect cell expression systems
Two ÄKTA FPLC Pure 25L systems AKTA FPLC Prime Plus
ÄKTA FPLC Purifier Biacore T200 SPR
Bioneer ExiProgen™ system Shaker incubators and fermenters
Cell disruptors High capacity centrifuges
Thermocyclers  

The PPG core laboratory has recently upgraded its surface plasmon resonance (SPR) system to a Biacore T200. The BiacoreT200 is a sensitive and versatile SPR instrument that is capable of studying most biomolecular interactions. The system provides label-free measurement of protein interactions with binding partners big and small. In the following video Eric Roush, Biacore Principal Application Scientist with GE Healthcare, talks about studies where the Biacore T200 has been used.

The laboratory has four FPLC systems, an AKTA
Purifier, two AKTA Pure 25L systems, and an AKTA Prime Plus.

 

 

 

The ÄKTA Pure 25L system allows for quick and easy customization due to the modular design of both the hardware and software. ÄKTA Pure allows unattended operations by efficiently automating the purification tasks. To address fast and efficient protein purification, ÄKTA Pure can be set up in different ways to handle multi-step purification. Both of the AKTA Pure systems can be connected to up to five columns, however all five columns cannot be run in parallel.

The ÄKTA Prime Plus system automates liquid handling, minimizing the problem of sample loss through spillage or misplaced tubes. Access to data on recovered target proteins is more straightforward than manual procedures allow. The system includes a pH monitor, as well as PrimeView software which enables the protein to be automatically and continually tracked in real time. Result files contain a complete record of each run, including method, curve data, and run log.

The AKTA purifier system is designed for high-performance purification and characterization of proteins, peptides, and nucleic acids.

A fully-automated Bioneer ExiProgen™ system is available. The protein synthesis and nucleic acid extraction system uses E. coli cell-free lysate for synthesis of protein from plasmid or PCR-amplified DNA. The crude protein extract then is further purified, using His-Tag engineered into the proteins. The ExiProgen can produce up to 100 mg of >90% pure protein in six hours, which saves time and provides quicker research results. This equipment is ideal for rush orders of small amounts of protein. It can extend to 96-well format and can produce up to 10 mg of protein. In addition, ExiProgen can also purify nucleic acids from several sources, which makes it an extremely versatile system.